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cy3 conjugated goat anti rat secondary antibody  (Jackson Immuno)


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    Jackson Immuno cy3 conjugated goat anti rat secondary antibody
    Cy3 Conjugated Goat Anti Rat Secondary Antibody, supplied by Jackson Immuno, used in various techniques. Bioz Stars score: 96/100, based on 781 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/donkey+anti+goat+cy3+secondary+antibody/Cy+3+AffiniPure+Donkey+Anti-Rat+IgG/pmc13050439-184-7-14
    Average 96 stars, based on 781 article reviews
    cy3 conjugated goat anti rat secondary antibody - by Bioz Stars, 2026-09
    96/100 stars

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    Related Articles

    Incubation:

    Article Title: A Comparative Study Evaluating the Impact of Physical Exercise on Disease Progression in a Mouse Model of Alzheimer’s Disease
    Article Snippet: Evidence suggests that physical exercise can serve as a preventive strategy against Alzheimer’s disease (AD).. In contrast, much less is known about the impact of exercise when it is introduced after cognitive deficits are established.. Using the TgCRND8 mouse model of amyloidosis, we compared the effects of exercise as an intervention strategy aimed at altering disease progression.

    Article Title: Sex-specific adipose tissue imprinting of regulatory T cells.
    Article Snippet: Glucose (1.75 g per kg of body weight) was injected intraperitoneally to mice fasted for 8 h. Blood samples were obtained from the tail tip at the indicated times, and blood glucose concentrations were measured using a handheld glucometer (Accu-Chek Performa, Roche). .. Intracellular IL-33 staining Intracellular IL-33 was detected by fixation and permeabilization using True-Nuclear Transcription Factor Buffer Set (BioLegend) as per the manufacturer’s instructions followed by incubation with a goat anti– IL-33 polyclonal primary antibody (no. AF326, R&D Systems) and a donkey anti-goat Cy3 secondary antibody (no. 705-166-147, Jackson Immunoresearch Laboratories). ..

    Article Title: Activin-mediated alterations of the fibroblast transcriptome and matrisome control the biomechanical properties of skin wounds
    Article Snippet: .. For immunofluorescence staining of cultured cells, coverslips were fixed in 4% PFA, blocked with 1% BSA, and incubated overnight with goat anti-fibronectin (#sc-6952; 1:500; Santa Cruz, Santa Cruz, CA) or goat anti-collagen I (#1310-01; 1:500; SouthernBiotech, Birmingham, AL) primary antibodies, followed by donkey anti-goat Cy3 secondary antibody (#705-165-147; 1:1000; Jackson ImmunoResearch, West Grove, PA), and counterstained with Hoechst 33342 (Sigma). .. For immunofluorescence staining of co-cultures, coverslips were fixed in 4% PFA, blocked with 10% normal donkey serum (#017-000-121; Jackson ImmunoResearch), and incubated overnight with rabbit anti-fibronectin (#ab2413 1:500; Abcam, Cambridge, UK) and goat anti-collagen I (1:500; SouthernBiotech), or with rabbit anti-periostin (#ab14041, 1:200; Abcam) and goat anti-asporin primary antibodies (#ab31303, 1:200; Abcam), followed by donkey anti-goat AlexaFluor 594 (#705-585-003) and donkey anti-rabbit AlexaFluor 647 (#711-605-152) secondary antibodies (1:1000; Jackson ImmunoResearch), and counterstained with DAPI (Sigma).

    Staining:

    Article Title: Sex-specific adipose tissue imprinting of regulatory T cells.
    Article Snippet: Glucose (1.75 g per kg of body weight) was injected intraperitoneally to mice fasted for 8 h. Blood samples were obtained from the tail tip at the indicated times, and blood glucose concentrations were measured using a handheld glucometer (Accu-Chek Performa, Roche). .. Intracellular IL-33 staining Intracellular IL-33 was detected by fixation and permeabilization using True-Nuclear Transcription Factor Buffer Set (BioLegend) as per the manufacturer’s instructions followed by incubation with a goat anti– IL-33 polyclonal primary antibody (no. AF326, R&D Systems) and a donkey anti-goat Cy3 secondary antibody (no. 705-166-147, Jackson Immunoresearch Laboratories). ..

    Article Title: Activin-mediated alterations of the fibroblast transcriptome and matrisome control the biomechanical properties of skin wounds
    Article Snippet: .. For immunofluorescence staining of cultured cells, coverslips were fixed in 4% PFA, blocked with 1% BSA, and incubated overnight with goat anti-fibronectin (#sc-6952; 1:500; Santa Cruz, Santa Cruz, CA) or goat anti-collagen I (#1310-01; 1:500; SouthernBiotech, Birmingham, AL) primary antibodies, followed by donkey anti-goat Cy3 secondary antibody (#705-165-147; 1:1000; Jackson ImmunoResearch, West Grove, PA), and counterstained with Hoechst 33342 (Sigma). .. For immunofluorescence staining of co-cultures, coverslips were fixed in 4% PFA, blocked with 10% normal donkey serum (#017-000-121; Jackson ImmunoResearch), and incubated overnight with rabbit anti-fibronectin (#ab2413 1:500; Abcam, Cambridge, UK) and goat anti-collagen I (1:500; SouthernBiotech), or with rabbit anti-periostin (#ab14041, 1:200; Abcam) and goat anti-asporin primary antibodies (#ab31303, 1:200; Abcam), followed by donkey anti-goat AlexaFluor 594 (#705-585-003) and donkey anti-rabbit AlexaFluor 647 (#711-605-152) secondary antibodies (1:1000; Jackson ImmunoResearch), and counterstained with DAPI (Sigma).

    Isolation:

    Article Title: Biomarkers of lesion severity in a rodent model of nonarteritic anterior ischemic optic neuropathy (rNAION)
    Article Snippet: .. PF-PBS-fixed retinas were isolated, and RGCs immunostained using a primary goat polyclonal anti-Brn3a antibody (Santa Cruz Biotechnology, Dallas, TX), followed by fluorescent labeling with a donkey anti-goat Cy3 secondary antibody (Jackson Immunoresearch, West Grove, PA), flat-mounted and stereologically counted as previously described, using a Nikon Eclipse E800 fluorescent microscope (Nikon, Melville NY) with motorized stage, driven by a stereological imaging package (StereoInvestigator, Ver 10.0; Microbrightfield Bioscience, Williston, VT). ..

    Article Title: Biomarkers of lesion severity in a rodent model of nonarteritic anterior ischemic optic neuropathy (rNAION).
    Article Snippet: .. PF-PBS-fixed retinas were isolated, and RGCs were immunostained using a primary goat polyclonal anti-Brn3a antibody (Santa Cruz Biotechnology, Dallas, TX), followed by fluorescent labeling with a donkey anti-goat Cy3 secondary antibody (Jackson Immunoresearch, West Grove, PA), flatmounted and stereologically counted as previously described, using a Nikon Eclipse E800 fluorescent microscope (Nikon, Melville NY) with motorized stage, driven by a stereological imaging package (StereoInvestigator, Ver 10.0; Microbrightfield Bioscience, Williston, VT). ..

    Article Title: Biomarkers of lesion severity in a rodent model of nonarteritic anterior ischemic optic neuropathy (rNAION)
    Article Snippet: .. PF-PBS-fixed retinas were isolated, and RGCs were immunostained using a primary goat polyclonal anti-Brn3a antibody (Santa Cruz Biotechnology, Dallas, TX), followed by fluorescent labeling with a donkey anti-goat Cy3 secondary antibody (Jackson Immunoresearch, West Grove, PA), flat-mounted and stereologically counted as previously described, using a Nikon Eclipse E800 fluorescent microscope (Nikon, Melville NY) with motorized stage, driven by a stereological imaging package (StereoInvestigator, Ver 10.0; Microbrightfield Bioscience, Williston, VT). ..

    Labeling:

    Article Title: Biomarkers of lesion severity in a rodent model of nonarteritic anterior ischemic optic neuropathy (rNAION)
    Article Snippet: .. PF-PBS-fixed retinas were isolated, and RGCs immunostained using a primary goat polyclonal anti-Brn3a antibody (Santa Cruz Biotechnology, Dallas, TX), followed by fluorescent labeling with a donkey anti-goat Cy3 secondary antibody (Jackson Immunoresearch, West Grove, PA), flat-mounted and stereologically counted as previously described, using a Nikon Eclipse E800 fluorescent microscope (Nikon, Melville NY) with motorized stage, driven by a stereological imaging package (StereoInvestigator, Ver 10.0; Microbrightfield Bioscience, Williston, VT). ..

    Article Title: Biomarkers of lesion severity in a rodent model of nonarteritic anterior ischemic optic neuropathy (rNAION).
    Article Snippet: .. PF-PBS-fixed retinas were isolated, and RGCs were immunostained using a primary goat polyclonal anti-Brn3a antibody (Santa Cruz Biotechnology, Dallas, TX), followed by fluorescent labeling with a donkey anti-goat Cy3 secondary antibody (Jackson Immunoresearch, West Grove, PA), flatmounted and stereologically counted as previously described, using a Nikon Eclipse E800 fluorescent microscope (Nikon, Melville NY) with motorized stage, driven by a stereological imaging package (StereoInvestigator, Ver 10.0; Microbrightfield Bioscience, Williston, VT). ..

    Article Title: Biomarkers of lesion severity in a rodent model of nonarteritic anterior ischemic optic neuropathy (rNAION)
    Article Snippet: .. PF-PBS-fixed retinas were isolated, and RGCs were immunostained using a primary goat polyclonal anti-Brn3a antibody (Santa Cruz Biotechnology, Dallas, TX), followed by fluorescent labeling with a donkey anti-goat Cy3 secondary antibody (Jackson Immunoresearch, West Grove, PA), flat-mounted and stereologically counted as previously described, using a Nikon Eclipse E800 fluorescent microscope (Nikon, Melville NY) with motorized stage, driven by a stereological imaging package (StereoInvestigator, Ver 10.0; Microbrightfield Bioscience, Williston, VT). ..

    Microscopy:

    Article Title: Biomarkers of lesion severity in a rodent model of nonarteritic anterior ischemic optic neuropathy (rNAION)
    Article Snippet: .. PF-PBS-fixed retinas were isolated, and RGCs immunostained using a primary goat polyclonal anti-Brn3a antibody (Santa Cruz Biotechnology, Dallas, TX), followed by fluorescent labeling with a donkey anti-goat Cy3 secondary antibody (Jackson Immunoresearch, West Grove, PA), flat-mounted and stereologically counted as previously described, using a Nikon Eclipse E800 fluorescent microscope (Nikon, Melville NY) with motorized stage, driven by a stereological imaging package (StereoInvestigator, Ver 10.0; Microbrightfield Bioscience, Williston, VT). ..

    Article Title: Biomarkers of lesion severity in a rodent model of nonarteritic anterior ischemic optic neuropathy (rNAION).
    Article Snippet: .. PF-PBS-fixed retinas were isolated, and RGCs were immunostained using a primary goat polyclonal anti-Brn3a antibody (Santa Cruz Biotechnology, Dallas, TX), followed by fluorescent labeling with a donkey anti-goat Cy3 secondary antibody (Jackson Immunoresearch, West Grove, PA), flatmounted and stereologically counted as previously described, using a Nikon Eclipse E800 fluorescent microscope (Nikon, Melville NY) with motorized stage, driven by a stereological imaging package (StereoInvestigator, Ver 10.0; Microbrightfield Bioscience, Williston, VT). ..

    Article Title: Biomarkers of lesion severity in a rodent model of nonarteritic anterior ischemic optic neuropathy (rNAION)
    Article Snippet: .. PF-PBS-fixed retinas were isolated, and RGCs were immunostained using a primary goat polyclonal anti-Brn3a antibody (Santa Cruz Biotechnology, Dallas, TX), followed by fluorescent labeling with a donkey anti-goat Cy3 secondary antibody (Jackson Immunoresearch, West Grove, PA), flat-mounted and stereologically counted as previously described, using a Nikon Eclipse E800 fluorescent microscope (Nikon, Melville NY) with motorized stage, driven by a stereological imaging package (StereoInvestigator, Ver 10.0; Microbrightfield Bioscience, Williston, VT). ..

    Imaging:

    Article Title: Biomarkers of lesion severity in a rodent model of nonarteritic anterior ischemic optic neuropathy (rNAION)
    Article Snippet: .. PF-PBS-fixed retinas were isolated, and RGCs immunostained using a primary goat polyclonal anti-Brn3a antibody (Santa Cruz Biotechnology, Dallas, TX), followed by fluorescent labeling with a donkey anti-goat Cy3 secondary antibody (Jackson Immunoresearch, West Grove, PA), flat-mounted and stereologically counted as previously described, using a Nikon Eclipse E800 fluorescent microscope (Nikon, Melville NY) with motorized stage, driven by a stereological imaging package (StereoInvestigator, Ver 10.0; Microbrightfield Bioscience, Williston, VT). ..

    Article Title: Biomarkers of lesion severity in a rodent model of nonarteritic anterior ischemic optic neuropathy (rNAION).
    Article Snippet: .. PF-PBS-fixed retinas were isolated, and RGCs were immunostained using a primary goat polyclonal anti-Brn3a antibody (Santa Cruz Biotechnology, Dallas, TX), followed by fluorescent labeling with a donkey anti-goat Cy3 secondary antibody (Jackson Immunoresearch, West Grove, PA), flatmounted and stereologically counted as previously described, using a Nikon Eclipse E800 fluorescent microscope (Nikon, Melville NY) with motorized stage, driven by a stereological imaging package (StereoInvestigator, Ver 10.0; Microbrightfield Bioscience, Williston, VT). ..

    Article Title: Biomarkers of lesion severity in a rodent model of nonarteritic anterior ischemic optic neuropathy (rNAION)
    Article Snippet: .. PF-PBS-fixed retinas were isolated, and RGCs were immunostained using a primary goat polyclonal anti-Brn3a antibody (Santa Cruz Biotechnology, Dallas, TX), followed by fluorescent labeling with a donkey anti-goat Cy3 secondary antibody (Jackson Immunoresearch, West Grove, PA), flat-mounted and stereologically counted as previously described, using a Nikon Eclipse E800 fluorescent microscope (Nikon, Melville NY) with motorized stage, driven by a stereological imaging package (StereoInvestigator, Ver 10.0; Microbrightfield Bioscience, Williston, VT). ..

    other:

    Article Title: Channel modulators
    Article Snippet: The retina was then carefully removed from the sclera and permeabilized via incubation in 0.5% Triton-X100 in PBS solution for 15 minutes at −80° C. Following thorough washing with PBS, free floating retinas were incubated overnight at 4° C. in goat anti-Brn3a primary antibody (SC-31984, Santa-Cruz Biotechnology, 1:100) in 2% horse serum and 2% Triton-X100 in PBS solution.

    Immunofluorescence:

    Article Title: Activin-mediated alterations of the fibroblast transcriptome and matrisome control the biomechanical properties of skin wounds
    Article Snippet: .. For immunofluorescence staining of cultured cells, coverslips were fixed in 4% PFA, blocked with 1% BSA, and incubated overnight with goat anti-fibronectin (#sc-6952; 1:500; Santa Cruz, Santa Cruz, CA) or goat anti-collagen I (#1310-01; 1:500; SouthernBiotech, Birmingham, AL) primary antibodies, followed by donkey anti-goat Cy3 secondary antibody (#705-165-147; 1:1000; Jackson ImmunoResearch, West Grove, PA), and counterstained with Hoechst 33342 (Sigma). .. For immunofluorescence staining of co-cultures, coverslips were fixed in 4% PFA, blocked with 10% normal donkey serum (#017-000-121; Jackson ImmunoResearch), and incubated overnight with rabbit anti-fibronectin (#ab2413 1:500; Abcam, Cambridge, UK) and goat anti-collagen I (1:500; SouthernBiotech), or with rabbit anti-periostin (#ab14041, 1:200; Abcam) and goat anti-asporin primary antibodies (#ab31303, 1:200; Abcam), followed by donkey anti-goat AlexaFluor 594 (#705-585-003) and donkey anti-rabbit AlexaFluor 647 (#711-605-152) secondary antibodies (1:1000; Jackson ImmunoResearch), and counterstained with DAPI (Sigma).

    Cell Culture:

    Article Title: Activin-mediated alterations of the fibroblast transcriptome and matrisome control the biomechanical properties of skin wounds
    Article Snippet: .. For immunofluorescence staining of cultured cells, coverslips were fixed in 4% PFA, blocked with 1% BSA, and incubated overnight with goat anti-fibronectin (#sc-6952; 1:500; Santa Cruz, Santa Cruz, CA) or goat anti-collagen I (#1310-01; 1:500; SouthernBiotech, Birmingham, AL) primary antibodies, followed by donkey anti-goat Cy3 secondary antibody (#705-165-147; 1:1000; Jackson ImmunoResearch, West Grove, PA), and counterstained with Hoechst 33342 (Sigma). .. For immunofluorescence staining of co-cultures, coverslips were fixed in 4% PFA, blocked with 10% normal donkey serum (#017-000-121; Jackson ImmunoResearch), and incubated overnight with rabbit anti-fibronectin (#ab2413 1:500; Abcam, Cambridge, UK) and goat anti-collagen I (1:500; SouthernBiotech), or with rabbit anti-periostin (#ab14041, 1:200; Abcam) and goat anti-asporin primary antibodies (#ab31303, 1:200; Abcam), followed by donkey anti-goat AlexaFluor 594 (#705-585-003) and donkey anti-rabbit AlexaFluor 647 (#711-605-152) secondary antibodies (1:1000; Jackson ImmunoResearch), and counterstained with DAPI (Sigma).



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    Jackson Immuno secondary anti goat cy3
    FFAR4 activation on ISCs influences lineage commitment toward secretory goblet cells in enteroids from normal-weight individuals. A Representative image of a whole-mount immunofluorescence staining of EdU (AlexaFluor 647, red) and FFAR4 <t>(CY3,</t> green) in an undifferentiated enteroid. B RT-qPCR analysis showing relative mRNA expression (efficiency −ΔCt ) of free fatty acid receptors (FFAR) 2 , 3 , and 4 in undifferentiated enteroids ( n = 6). C Principal component analysis-plot showing the total variance in the gene expression profile of TUG891-treated (10 µM) and untreated enteroids ( n = 8). D Volcano plot depicting the top DEGs between TUG891-treated (10 µM) and untreated enteroids ( n = 8). The dotted horizontal line represents a Benjamini-Hochberg corrected P-value of 0.05 (FDR), and the vertical lines indicate a minimum fold-change of 1.4 for the most DEGs. E Violin plot showing downregulated DEGs between TUG891-treated (10 µM) and untreated enteroids ( n = 8), with gene enrichment and KEGG analyses identifying associated functions. F Violin plots illustrating the effect of 10 µM TUG891 on genes identifying goblet cell subtypes from the small intestine ( n = 8), as determined from published human intestinal single-cell RNA sequencing data . G Representative image on the effect of TUG891 on fucosylation, using UEA1 in TUG891-treated (10 µM) and untreated enteroids (red: UEA1; blue: nuclei, DAPI). H Quantification of UEA1-binding fucosylated glycans based on mean fluorescence intensity (585 nm) per enteroid surface area following TUG891 treatment (10 µM) ( n = 4). Data are presented as mean ± SEM. n represents the number of enteroid cultures established from normal-weight individuals. * P < 0.05, *** P < 0.001 vs. control. GO Gene Ontology; KEGG Kyoto Encyclopedia of Genes and Genomes; Wnt Wingless-type integration site; BMP Bone Morphogenetic Protein; GCs Goblet Cells
    Secondary Anti Goat Cy3, supplied by Jackson Immuno, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    FFAR4 activation on ISCs influences lineage commitment toward secretory goblet cells in enteroids from normal-weight individuals. A Representative image of a whole-mount immunofluorescence staining of EdU (AlexaFluor 647, red) and FFAR4 <t>(CY3,</t> green) in an undifferentiated enteroid. B RT-qPCR analysis showing relative mRNA expression (efficiency −ΔCt ) of free fatty acid receptors (FFAR) 2 , 3 , and 4 in undifferentiated enteroids ( n = 6). C Principal component analysis-plot showing the total variance in the gene expression profile of TUG891-treated (10 µM) and untreated enteroids ( n = 8). D Volcano plot depicting the top DEGs between TUG891-treated (10 µM) and untreated enteroids ( n = 8). The dotted horizontal line represents a Benjamini-Hochberg corrected P-value of 0.05 (FDR), and the vertical lines indicate a minimum fold-change of 1.4 for the most DEGs. E Violin plot showing downregulated DEGs between TUG891-treated (10 µM) and untreated enteroids ( n = 8), with gene enrichment and KEGG analyses identifying associated functions. F Violin plots illustrating the effect of 10 µM TUG891 on genes identifying goblet cell subtypes from the small intestine ( n = 8), as determined from published human intestinal single-cell RNA sequencing data . G Representative image on the effect of TUG891 on fucosylation, using UEA1 in TUG891-treated (10 µM) and untreated enteroids (red: UEA1; blue: nuclei, DAPI). H Quantification of UEA1-binding fucosylated glycans based on mean fluorescence intensity (585 nm) per enteroid surface area following TUG891 treatment (10 µM) ( n = 4). Data are presented as mean ± SEM. n represents the number of enteroid cultures established from normal-weight individuals. * P < 0.05, *** P < 0.001 vs. control. GO Gene Ontology; KEGG Kyoto Encyclopedia of Genes and Genomes; Wnt Wingless-type integration site; BMP Bone Morphogenetic Protein; GCs Goblet Cells
    Cy3 Conjugated Donkey Anti Goat Igg (H + L) Secondary Antibody Gb21404, supplied by Servicebio Inc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Image Search Results


    FFAR4 activation on ISCs influences lineage commitment toward secretory goblet cells in enteroids from normal-weight individuals. A Representative image of a whole-mount immunofluorescence staining of EdU (AlexaFluor 647, red) and FFAR4 (CY3, green) in an undifferentiated enteroid. B RT-qPCR analysis showing relative mRNA expression (efficiency −ΔCt ) of free fatty acid receptors (FFAR) 2 , 3 , and 4 in undifferentiated enteroids ( n = 6). C Principal component analysis-plot showing the total variance in the gene expression profile of TUG891-treated (10 µM) and untreated enteroids ( n = 8). D Volcano plot depicting the top DEGs between TUG891-treated (10 µM) and untreated enteroids ( n = 8). The dotted horizontal line represents a Benjamini-Hochberg corrected P-value of 0.05 (FDR), and the vertical lines indicate a minimum fold-change of 1.4 for the most DEGs. E Violin plot showing downregulated DEGs between TUG891-treated (10 µM) and untreated enteroids ( n = 8), with gene enrichment and KEGG analyses identifying associated functions. F Violin plots illustrating the effect of 10 µM TUG891 on genes identifying goblet cell subtypes from the small intestine ( n = 8), as determined from published human intestinal single-cell RNA sequencing data . G Representative image on the effect of TUG891 on fucosylation, using UEA1 in TUG891-treated (10 µM) and untreated enteroids (red: UEA1; blue: nuclei, DAPI). H Quantification of UEA1-binding fucosylated glycans based on mean fluorescence intensity (585 nm) per enteroid surface area following TUG891 treatment (10 µM) ( n = 4). Data are presented as mean ± SEM. n represents the number of enteroid cultures established from normal-weight individuals. * P < 0.05, *** P < 0.001 vs. control. GO Gene Ontology; KEGG Kyoto Encyclopedia of Genes and Genomes; Wnt Wingless-type integration site; BMP Bone Morphogenetic Protein; GCs Goblet Cells

    Journal: Stem Cell Research & Therapy

    Article Title: FFAR4 drives stem cell fate toward lipid- and iron-storing enterocytes protecting human enteroids from ferroptosis

    doi: 10.1186/s13287-025-04840-2

    Figure Lengend Snippet: FFAR4 activation on ISCs influences lineage commitment toward secretory goblet cells in enteroids from normal-weight individuals. A Representative image of a whole-mount immunofluorescence staining of EdU (AlexaFluor 647, red) and FFAR4 (CY3, green) in an undifferentiated enteroid. B RT-qPCR analysis showing relative mRNA expression (efficiency −ΔCt ) of free fatty acid receptors (FFAR) 2 , 3 , and 4 in undifferentiated enteroids ( n = 6). C Principal component analysis-plot showing the total variance in the gene expression profile of TUG891-treated (10 µM) and untreated enteroids ( n = 8). D Volcano plot depicting the top DEGs between TUG891-treated (10 µM) and untreated enteroids ( n = 8). The dotted horizontal line represents a Benjamini-Hochberg corrected P-value of 0.05 (FDR), and the vertical lines indicate a minimum fold-change of 1.4 for the most DEGs. E Violin plot showing downregulated DEGs between TUG891-treated (10 µM) and untreated enteroids ( n = 8), with gene enrichment and KEGG analyses identifying associated functions. F Violin plots illustrating the effect of 10 µM TUG891 on genes identifying goblet cell subtypes from the small intestine ( n = 8), as determined from published human intestinal single-cell RNA sequencing data . G Representative image on the effect of TUG891 on fucosylation, using UEA1 in TUG891-treated (10 µM) and untreated enteroids (red: UEA1; blue: nuclei, DAPI). H Quantification of UEA1-binding fucosylated glycans based on mean fluorescence intensity (585 nm) per enteroid surface area following TUG891 treatment (10 µM) ( n = 4). Data are presented as mean ± SEM. n represents the number of enteroid cultures established from normal-weight individuals. * P < 0.05, *** P < 0.001 vs. control. GO Gene Ontology; KEGG Kyoto Encyclopedia of Genes and Genomes; Wnt Wingless-type integration site; BMP Bone Morphogenetic Protein; GCs Goblet Cells

    Article Snippet: After washing, enteroids were stained during 2 h with a secondary antibody CY3 donkey anti-goat (1:800; 715-165-150, Jackson ImmunoResearch).

    Techniques: Activation Assay, Immunofluorescence, Staining, Quantitative RT-PCR, Expressing, Gene Expression, RNA Sequencing, Binding Assay, Fluorescence, Control